Correlation between Klotho changes and calcium-phosphate concentration in the serum at early stages of multiple sclerosis

Mehdi Amiri, Mohammad Hossein Harirchian, Saman Esmaeilnejad, Bahaadin Siroos, Mohammad Sajad Emami-Aleagha

DOI: 10.22122/cdj.v5i1.231

Abstract


BACKGROUND: There are several studies indicating that an anti-aging protein, namely Klotho protein, participates in the regulation of calcium and phosphate metabolism. In addition, we showed that Klotho protein was involved in the pathogenesis of multiple sclerosis (MS). Hence, we hypothesized that Klotho protein changes in patients with multiple sclerosis might lead to alteration of calcium and phosphate metabolism. Accordingly, the aim of the present study was to evaluate the alteration of calcium and phosphate levels together with the concentration of Klotho protein in the serum of patients with multiple sclerosis.

METHODS: In this case-control study, 14 patients with newly diagnosed relapsing-remitting multiple sclerosis (RRMS) along with 14 control individuals with noninflammatory neurological disorders were enrolled. The serum concentrations of Klotho protein, calcium, and phosphate were measured in serum of participants using commercial kits. The data were analyzed at the significant level of P < 0.050.

RESULTS: There were no significant changes in serum concentrations of Klotho protein, and phosphate in patients with multiple sclerosis when compared to controls. However, the serum calcium concentration was significantly lower than the control group. Regarding patients with multiple sclerosis, there was a significant positive correlation between changes in serum concentrations of Klotho protein and calcium (r = 0.604, P = 0.022), whereas the other correlations were not statistically significant.

CONCLUSION: To our knowledge, this is the first study demonstrating a positive correlation between serum concentrations of secretory Klotho protein and calcium in patients with multiple sclerosis.


Keywords


Klotho Protein; Calcium; Phosphorus; Multiple sclerosis

Full Text:

PDF

References


Kuro-o M. Klotho as a regulator of fibroblast growth factor signaling and phosphate/calcium metabolism. Curr Opin Nephrol Hypertens 2006; 15(4): 437-41.

Imura A, Tsuji Y, Murata M, Maeda R, Kubota K, Iwano A, et al. alpha-Klotho as a regulator of calcium homeostasis. Science 2007; 316(5831): 1615-8.

Li SA, Watanabe M, Yamada H, Nagai A, Kinuta M, Takei K. Immunohistochemical localization of Klotho protein in brain, kidney, and reproductive organs of mice. Cell Struct Funct 2004; 29(4): 91-9.

Kuro-o M. Endocrine FGFs and Klothos: Emerging concepts. Trends Endocrinol Metab 2008; 19(7): 239-45.

Kuro-o M. Klotho in health and disease. Curr Opin Nephrol Hypertens 2012; 21(4): 362-8.

Emami Aleagha MS, Siroos B, Ahmadi M, Balood M, Palangi A, Haghighi AN, et al. Decreased concentration of Klotho in the cerebrospinal fluid of patients with relapsing-remitting multiple sclerosis. J Neuroimmunol 2015; 281: 5-8.

Ahmadi M, Emami Aleagha MS, Harirchian MH, Yarani R, Tavakoli F, Siroos B. Multiple sclerosis influences on the augmentation of serum Klotho concentration. J Neurol Sci 2016; 362: 69-72.

Karami M, Mehrabi F, Allameh A, Pahlevan KM, Amiri M, Emami Aleagha MS. Klotho gene expression decreases in peripheral blood mononuclear cells (PBMCs) of patients with relapsing-remitting multiple sclerosis. J Neurol Sci 2017; 381: 305-7.

Polman CH, Reingold SC, Banwell B, Clanet M, Cohen JA, Filippi M, et al. Diagnostic criteria for multiple sclerosis: 2010 revisions to the McDonald criteria. Ann Neurol 2011; 69(2): 292-302.

Ellidag HY, Yilmaz N, Kurtulus F, Aydin O, Eren E, Inci A, et al. The Three Sisters of Fate in Multiple Sclerosis: Klotho (Clotho), Fibroblast Growth Factor-23 (Lachesis), and Vitamin D (Atropos). Ann Neurosci 2016; 23(3): 155-61.


Refbacks

  • There are currently no refbacks.


Creative Commons Attribution-NonCommercial 4.0

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.