16S Ribosomal Ribonucleic Acid Gene Sequencing for Identification of Multiple Drug-Resistant Bacteria
16S rRNA Gene Sequencing of Bacteria
Keywords:
Pseudomonas Aeruginosa, Acinetobacter, Enterobacteriaceae, 16S ribosomal ribonucleic acidAbstract
Introduction: Combining 16S ribosomal ribonucleic acid (16S rRNA) gene sequence analysis with the assessment of antibiotic resistance genes can play a key role in identifying drug-resistant bacteria and optimizing treatment management. This study aimed to identify 16S rRNA gene sequencing for the identification of multiple drug-resistant (MDR) bacteria among different samples.
Method: This epidemiological-descriptive study was conducted from September to December 2023 with bacteria isolated from the personnel and environment in Velayat Hospital, Qazvin City, Iran. The antibiotic resistance pattern was performed according to the Clinical and Laboratory Standards Institute (CLSI). Polymerase chain reaction (PCR) was performed using specific primers for 16S rRNA. Statistical Package for the Social Sciences (SPSS) 22 software, descriptive statistics, and Chi-square test (X2) were used for data analysis.
Result: Of the 186 collected samples, the highest and lowest isolates were related to P. aeruginosa (39.78%) and Enterobacter (9.67%). 74.86% of the total isolates produced the Extended Spectrum Beta-Lactamase (ESBL), and 24.59% of the isolates were MDR. There was no significant relationship between ESBL-producing and MDR isolates with sample types (p>0.05); both types of isolates (isolates from the environment and personnel) showed ESBL-producing and MDR. Deoxyribonucleic acid (DNA) sequences in isolates were similar to the template sequence of bacteria. There was 94.70% genetic similarity between the strains isolated from personnel and the environment.
Conclusion: Isolates producing ESBL and MDR had a high abundance among bacterial isolates. High genetic similarity between bacteria indicates the distribution of genetically similar strains between personnel and the hospital environment.
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