http://cdjournal.muk.ac.ir/index.php/cdj The Chronic Diseases Journal is a quarterly peer-reviewed scientific journal published by Kurdistan University of Medical Sciences since 2013. This journal has the certification of Medical Journals Commission of Iranian Ministry of Health and Medical Education as a S IENTIFIC-RESEARCH journal from 2019 (No. 1392/04/20-15/92/4508). In each year, this journal publishes about 32 to 40 research and 2 to 3 review articles. Time from submission to first decision is 2 days, time from submission to final decision is 3 weeks, and time from acceptance to publication is 2-10 weeks. Vesnu Publications en-US Chronic Diseases Journal 2588-7297 http://cdjournal.muk.ac.ir/index.php/cdj/article/view/952 BACKGROUND: Vaginal candidiasis is one of the most important fungal diseases in humans. Butternut squash (B. squash) has antifungal effects. This study investigated the effect of alcoholic extracts of B. squash on Candida albicans (C. albicans). METHODS: In this laboratory experiment, B. squash using convenient sampling was collected from Babol City, Iran, in 2019. Determining the sensitivity of C. albicans to alcoholic extracts was tested using the disk, well, and minimum fungicidal concentration (MFC) methods and through determining the minimum inhibitory concentrations (MICs). Gas chromatography (GC) was used to determine the effective substances of the chemical compounds of B. squash extract. SPSS software, two-way analysis of variance, multiple comparisons, and independent samples t-test were used for data analysis. RESULTS: Around the disk with 70 g/ml of ethanol extract (10 mm) and methanol (11 mm), and the wells with 110 mg/ml of methanol extract (17 mm), the average growth halo was greater. The size of the non-growth halo and the increasing trend of halo diameter between different alcoholic groups increased with increasing extract concentrations, but this increase was not significant (P < 0.05). In all 3 extracts, MIC was observed in 5-9 tubes and MFC in 3-9. The highest chemical composition was related to 5-hydroxymethylfurfural with 83.62%. CONCLUSION: Based on the results of this study, B. squash alcoholic extracts effectively inhibit C. albicans and can be used as a promising tool to control or treat fungal diseases. Azam Ghorbannia-Delaver Issa Gholampour-Azizi Atefeh Bozorgi Faezeh Hassanzadeh Copyright (c) 2024 Chronic Diseases Journal https://creativecommons.org/licenses/by-nc/4.0 2024-12-01 2024-12-01 241 248 10.22122/cdj.v12i4.952